RE: yellow rbc
Checked the pH of your formalin lately? Might be hematin (or formalin)
pigment. Try immersing the deparaffinized slide in 70 % ethanol containing 3
mL of ammonium hydroxide for 30 min - 3 hours at room temp. Rinse well with
water. Rinse with 1 % acetic acid(aq). Rinse with water again and proceed
with the staining.
Good luck, Donna Montague
From: Mark Schrenzel [mailto:firstname.lastname@example.org]
Sent: Wednesday, March 21, 2001 1:17 PM
Subject: yellow rbc
We are getting yellow staining of red blood cells in formalin-fixed
mammalian tissues. Does anybody have any experiences or ideas about this?
Thanks very much.
Zoological Society of San Diego
Center for Reproduction of Endangered Species
P.O. Box 120-551
San Diego, California 92112
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