This is exactly why we do not do any HIER for bone IHC.  Heat will make the
componets of the section fall off selectively, we often lost the cartilage
and since cartilage was what we were studing it was really bad news.  I use
enzyme digestion only for IHC pretreatment on bone samples.
Patsy Ruegg

		-----Original Message-----
		From:	Terry.Marshall@rgh-tr.trent.nhs.uk
[mailto:Terry.Marshall@rgh-tr.trent.nhs.uk]
		Sent:	Wednesday, March 28, 2001 8:13 AM
		To:	info@instrumedics.com; HistoNet@pathology.swmed.edu
		Subject:	RE: sections falling off slide

		Bernice wrote:

		"Kate and I had a long discussion and we think that
somewhere in the antigen
		retrieval process, which is done in a pressure cooker,  the
bone is being
		lost. Perhaps  the acid buffer solution used in the pressure
cooking may be
		dissolving the bone. The marrow remains on the slide!"

		Odd isn't it. You boil bone to make glue!

		Terry L Marshall
		Histopathologist
		Rotherham General Hospital, Yorkshire