I use Solochrome azurine as well, much more sensitive and easy than the
aluminon stain.  Decalcified paraffin embedded sections are not good for AL
staining the decal will also remove the aluminum deposits.
Patsy Ruegg

		-----Original Message-----
		From:	t.hacker@har.mrc.ac.uk
[mailto:T.Hacker@har.mrc.ac.uk]
		Sent:	Thursday, March 29, 2001 12:31 AM
		To:	Marlene Crissey
		Cc:	'Histonet'
		Subject:	Re: aluminum staining

		Date sent:      	Wed, 28 Mar 2001 14:42:49 -0500
		From:           	Marlene Crissey <mcc12@cornell.edu>
		Subject:        	aluminum staining
		To:             	HistoNet@pathology.swmed.edu

		> Does anyone have a reliable staining method to demonstrate
aluminum in 
		> paraffin (not plastic) embedded tissues?
		> thank you in advance for the help-
		>
		A simple and effective method I have used is the solochrome 
		azurine technique (1972) found in Bancroft and Stevens
(Theory 
		and Practise of Histological Techniques), also for
beryllium.
		Terry.
		Terry Hacker,
		Medical Research Council,
		Harwell,
		Didcot,
		Oxfordshire, OX11 ORD
		01235 834393 x360