Wow, I have never heard of the sections falling off with this system.  Are
you putting the tape on their coated slides?
Patsy Ruegg

		-----Original Message-----
		From:	Kate_E_Rhodes@sbphrd.com
[mailto:Kate_E_Rhodes@sbphrd.com]
		Sent:	Tuesday, March 27, 2001 7:31 AM
		To:	HistoNet@pathology.swmed.edu
		Subject:	Paraffin Sectioning Aid cutting bone???

		Hello Histonet-We are currently cutting human bone with the
Instrumedics
		Paraffin Sectioning Aid and have experienced problems with
the sections
		falling off while doing an immunohistochemical protocol for
Cat-S requiring
		antigen retrieval. The antigen retrieval method we are now
using is
		Decloaker in the pressure cooker for 5 min., warm for 10
min. and then cool
		for 20 min. Could anyone offer suggestions for an alternate
antigen
		retrieval using this sectioning aid or any other method to
prevent section
		loss??

		Thank you,
		Kate Rhodes
		GSK
		610-270-7340
		FAX 610-270-7202