Hi Tracey, just take the slide back to 0.5 or 1% acid alcohol and remove the
desired amount of Hx staining from the section and blue up in bicarb or
whatever, then dehydrate and mount. David.

David Taylor
Laboratory Manager
Drs King & Mower
Adelaide, Australia


-----Original Message-----
From: Tracey Gunn [mailto:tracey.gunn@stonebow.otago.ac.nz]
Sent: Thursday, 29 March 2001 08:02
To: Histonet
Subject: Destain haematoxylin, not DAB


Hello,

A client wishes to remove haematoxylin from an immuno slide stained with
DAB.  She has left the slide in haematoxylin too long, and now the DAB is
extremely hard to see.  Is there any way we can remove the haematoxylin
without removing the DAB?

Thanks,
Tracey Gunn,
Histology Service Unit,
Otago University,
Dunedin,
NEW ZEALAND.