Hello Histonet-We are currently cutting human bone with the Instrumedics
Paraffin Sectioning Aid and have experienced problems with the sections
falling off while doing an immunohistochemical protocol for Cat-S requiring
antigen retrieval. The antigen retrieval method we are now using is
Decloaker in the pressure cooker for 5 min., warm for 10 min. and then cool
for 20 min. Could anyone offer suggestions for an alternate antigen
retrieval using this sectioning aid or any other method to prevent section
loss??

Thank you,
Kate Rhodes
GSK
610-270-7340
FAX 610-270-7202