Immuno on freezing microtome sections.

From:Neuropathology <>

I 've been asked to cut 50 micron freezing microtome sections of a skin
biopsy and immunostain with PGP 9.5. I'm happy (??!!) to cut the sections
but would welcome any advice on the immunostaining. Is it better to free
float the sections through the reagents or mount the sections onto slides,
air dry and then stain? Is immunofluorescense best or can I use the standard
immuno method as used for cryostat sections?

Bob Quilty
Dept of Neuropathology
Frenchay Hospital

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