GFP mice and how to fix?

From:Kim Kusser <>

Hi all,

I need some suggestions on how to do the following:

We have the GFP mice.... Anyways, we are going to take the cells from the GFP mice, and inject them into non-GFP mice to follow. Now, the questions is...what is the best way to fix organs when sacrificing the animals as to preserve the GFP protein? I will also want to do visualize other antigens with fluoresence. I usually just freeze organs in OCT on LN2.

Any ideas?


Kim Kusser

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