GFP mice and how to fix?
From: | Kim Kusser <kkusser@trudeauinstitute.org> |
Hi all,
I need some suggestions on how to do the following:
We have the GFP mice.... Anyways, we are going to take the cells from the GFP mice, and inject them into non-GFP mice to follow. Now, the questions is...what is the best way to fix organs when sacrificing the animals as to preserve the GFP protein? I will also want to do visualize other antigens with fluoresence. I usually just freeze organs in OCT on LN2.
Any ideas?
Thanks
Kim Kusser
kkusser@trudeauinstitute.org
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