(Fwd) Re: LACMOID
|From:||RUSS ALLISON <Allison@Cardiff.ac.uk>|
Subject: Re: LACMOID
Send reply to: ALLISON@CARDIFF.AC.UK
Date sent: Fri, 16 Mar 2001 08:07:18 GMT0BST
Floyd Green in the Sigma-Aldrich Handbook of Stains, Dyes and
Indicators lists Lacmoid.
Brief info: - used as indicator and in plant histology.
More info for dye buffs! - and I hope I do not transgress the
An oxazine dye made by heating resorcinol, sodium nitrite and
water (100:5:5)in an oil bath until all of the ammonia emanating
from the reaction has been depleted. The resulting melt is
digested in hot water and a crude product is precipitated from the
filtrate by adjusting the pH to approx. 5.8 with hydrochloric acid.
Further purification is accomplished by an ethanol recrystallisation.
- and you think you've got Health & Safety ptoblems!!!
It is soluble in methanol, ethanol acetic acid and acetone.
for plant material containing callose (not a typo), as a counterstain
to haematoxylin for general plant tissue, as a neatral counterstain
used with haematoxylin for phloem and, to demonstrate callose in
pollen tubes with Martius yellow.
No reference to human tissue oocytes or anything else - except as
an acid/base indicator.
Incidentally - Floyd Green's is a smashing book!
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