"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk>
<html>
Aiden,<br>
<x-tab> </x-tab>Have to
disagree with your interpretation. When you do the sums 38.1% use a
commercial diluent, while the sensible 61.9% make their own.<br>
Ian.<br>
<br>
<br>
<blockquote type=cite class=cite cite>Date: Tue, 27 Mar 2001 15:23:16
+1200<br>
From: Aidan Schurr <Aidan.Schurr@hvh.co.nz><br>
Subject: immunodiluent survey results.<br>
To: histonet@pathology.swmed.edu<br>
<br>
Hi all<br>
<br>
Survey results as follows:<br>
<br>
Diluent
# responses<br>
0.1% BSA in
PBS:
3<br>
1% BSA in
PBS:
4<br>
+ tween or
triton-X: 3<br>
+
Azide
2<br>
Commercial
8<br>
PBS
1<br>
<br>
So it seems the majority of us use commercial diluent, followed closely
by BSA in PBS (of various concentrations - I find myself wondering if the
dilution is important at all!)<br>
<br>
Interesting!<br>
<br>
Aidan<br>
New Zealand<br>
<br>
<br>
<br>
__<br>
<br>
aidan schurr b.m.l.sc<br>
section head, histology<br>
hutt valley health<br>
lower hutt<br>
new zealand<br>
<br>
aidan.schurr@hvh.co.nz<br>
++64 4 570 9173</blockquote>
<x-sigsep><p></x-sigsep>
<font color="#0000FF">Dr. Ian Montgomery,<br>
West Medical Building,<br>
University of Glasgow,<br>
Glasgow,<br>
G12 8QQ.<br>
Tel: 0141 339 8855. Extn:6602.<br>
Fax: 0141 330 2923<br>
e-mail: ian.montgomery@bio.gla.ac.uk</font></html>