|From:||"Su, Phy-Huynh" <email@example.com>|
May I ask a question about methyl benzoate, please.
I have received a protocol to fix tissues in acetone, at -80 degree C with
few changes, then bring the tissues up to RT in a fresh one, then going into
methyl benzoate before going into xylene, then paraffin. I understand that
methyl benzoate is a clearing agent, as xylene, but is it really necessary?
Does it act as another clearing agent here or does it have any other
affects? Can I just go directly to Xylene as routine processing? How about
putting the tissues into 50%, 70%, 100% alcohol? Will this deterioate the
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