Hi all,

The use of triphenyltetrazolium chloride (TTC) solution is common for
demonstrating infarcted heart tissue in gross specimens. Usually the whole
formalin fixed specimen is placed in the TTC before processing. The dye
imparts a red colour to the unaffected myocardium.

Does anyone have experience with using this method?
Does it interfere with subsequent tissue processing?
Can tissue treated this way using for H&E and Immunohistochemistry staining?

I would appreciate talking to anyone who has using TTC in muscle staining
for Myocardial infarcts.

Thanks.

Todd


Todd Walker
CT(ASC)., CFIAC., Ph.D.

Lecturer in Cytology / Histology
School of Biomedical Sciences
Charles Sturt University
Wagga Wagga NSW 2650
AUSTRALIA.

Tel: 02-69332541
Fax: 02-69332587

E-mail: towalker@csu.edu.au


-----Original Message-----
From:	james.zimmerman@pharma.Novartis.com
[mailto:james.zimmerman@pharma.Novartis.com]
Sent:	Friday, 16 March 2001 6:06
To:	Histonet@pathology.swmed.edu
Subject:	Double Staining

     Does anyone have any experience with an H&E plus  an
immunohistochemical stain with a DAB chromagen .

                                              Jim Zimmerman