Tetracycline Labeling of Bone

From:George Spurr <gspurr@interchange.ubc.ca>

Histoneters,
    I  am labeling bone in Vivo with Tetracycline, Calcein and Yylenol
Orange at different Time intervals. I  process and cut the Undecalcified
bone in plastic.

My question is This:
        Would the three labels dissapear in decalcified bone?
I know they label calcium (in vivo), but would the signal not still be
there if you removed the calcium later.

--
George Spurr,  RT, RLAT

UBC Department of Anatomy
     and       Wax - It  HISTOLOGY SERVICES
Phone:  (604) 822-1595
Fax:    (604) 822-2316
Email: gspurr@interchg.ubc.ca
http://www.wax-it.org





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