Re: vimentin control for fixation in IHC

From:Jan Shivers <>

I have never had a problem with Vimentin, so I have not understood what
people were talking about when saying they used it to test for
immunoreactivity.  Let's face it.... in the veterinary world samples can sit
in formalin for days, weeks, months, before they're blocked up and stained
with IHC.  Worse yet, samples can sit out in the field autolyzing for days
before a veterinarian can get samples into formalin.

I have only used clone V9.  Have I merely hit on the magic clone for the
extreme range of fixation times (or lack thereof) on which I work?

Jan Shivers
Univ. of Minnesota Veterinary Diagnostic Lab
St. Paul, MN

----- Original Message -----
From: "C & J Haley" <>
To: "HistoNet Server" <>; "Hagerty, Marjorie A."
Sent: Wednesday, March 07, 2001 9:40 PM
Subject: vimentin control for fixation in IHC

> We use the Vimentin (V9 clone)  from Biogenex.   I would like to expand
> this discussion.  I have never felt the vimentin was the best indicator
> of immunoreactivity.  Maybe if you were only working with intermediate
> filaments it would be okay.  We all know that Ki67, ER, PR, Her2, and
> about any CD marker would not survive the abuse that Vimentin does.  So
> what are the opinions on Vimentin's relevance to tissue
> immunoreactivity?
> Just curious,
> Jane Haley

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