IF your bm bxs are 2-3mm in thickness then your processing times could 
very well be your problem. They need a little longer in 100% 
ethanol,xylene, and parraffin than cores less than 2mm thick. 20 minutes 
total in 100% ethanol 25 in xylene 20-25 in paraffin.

Rena Fail
Medical University of SC
Charleston.SC


t 08:34 AM 3/3/01 -0500, you wrote:
>Having trouble with bone marrows, been using a short processing program (5
>minutes in each station)  don't think that's the problem.  Great vacuoles and
>separation is happening around the cells some how, have tried many solutions
>but still the problem persists, looking for some new ideas.
>
>Bernadette Weston HT
>Barberton Citizen's  Hospital
>Barberton, OH
>BernaW@aol.com