Re: soaking blocks
|From:||Cynthia Tily <email@example.com>|
I also worked in a lab that recommended soaking overnite. We worked mainly
with murine tissues and it turned out they were so overprocessed that I
usually did have to soak that long to get a decent section.Of course, I had
no control over the processing schedule, so I just had to make do.
One word of caution-neural tissues will swell out of the blocks if left
overnite,they may need only an hour or two on the ice.
Coll of Vet Med
----- Original Message -----
From: Rick Haverland <firstname.lastname@example.org>
Sent: Wednesday, March 14, 2001 7:18 AM
Subject: soaking blocks
> I would like to get some input on the appropriate amount of time to
> soak/chill animal tissue before cutting it. In my experience, properly
> processed tissue takes a very short amount of time before it is ready to
> cut. The lab I am currently working for is recommending soaking blocks
> overnight on ice in a refrigerator. I have never heard of this and am
> concerned about tissue damage. Thanks for your help.
> Bly Haverland
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