I soak both rat and mouse tissue at least an hour, not more than an hour
and a half.
Gina Sennello
Gilead Sciences




                                                                                            
                    Rick                                                                    
                    Haverland            To:     histonet@pathology.swmed.edu               
                    <corycody@msn        cc:                                                
                    .com>                Subject:     soaking blocks                        
                                                                                            
                    03/14/01                                                                
                    05:18 AM                                                                
                                                                                            
                                                                                            




I would like to get some input on the appropriate amount of time to
soak/chill animal tissue before cutting it. In my experience, properly
processed tissue takes a very short amount of time before it is ready to
cut. The lab I am currently working for is recommending soaking blocks
overnight on ice in a refrigerator. I have never heard of this and am
concerned about tissue damage. Thanks for your help.

Bly Haverland
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