Re: procedure staining for bone and cartilage
| From: | RichardWHorobin@aol.com |
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Andi asked:
> I was wondering if anybody does or has done a stain for bone and cartilage
> on mouse pups using Alizarin Red S and Alcian Blue. The investigator
> brought a great picture in a book and asked me to do this stain but I can't
> .
More great pictures, and complete protocols, for rat pups and fish larvae
respectively in the following:
An automated double staining procedure for bone and cartilage.
Miller DM & Tarpley J. 1996. Biotechnic & Histochemistry. 71: 79-83
Detection of mineralized structures in early stages of development of
marine
Teleostei using a modified alcian blue-alizarin red double staining
technique for
bone & cartilage.
Gavaia PJ, Sarasquete C & Cancela ML. 1999. Biotech Histochem. 75: 79-84
Incidentally, I do recommend Biotechnic and Histochemistry, the journal of
the Biological Stain Commission. Its full of neat methods and ideas, its good
looking, and its cheap - and we all know HistoNetters are deeply into
bargains! See its website at:
www.louisville.edu/journal/biotech-histochem
Ah - Being of a sceptical disposition I've just checked the site, and bits of
it are damaged! Its pretty though, but the editor can be contacted by email
at:
biotech-histochem@louisville.edu
And yes of COURSE I'm biased - being on the editorial board
Bye - Richard Horobin
Institute of Biomedical & Life Sciences, University of Glasgow
T direct 01796-474 480 --- E RichardWHorobin@aol.com
"What should we expect? Everything."
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<HTML><FONT FACE=arial,helvetica><FONT SIZE=2>Andi asked:
<BR>
<BR><BLOCKQUOTE TYPE=CITE style="BORDER-LEFT: #0000ff 2px solid; MARGIN-LEFT: 5px; MARGIN-RIGHT: 0px; PADDING-LEFT: 5px">I was wondering if anybody does or has done a stain for bone and cartilage
<BR>on mouse pups using Alizarin Red S and Alcian Blue. The investigator
<BR>brought a great picture in a book and asked me to do this stain but I can't
<BR>find a procedure</BLOCKQUOTE>.</FONT><FONT COLOR="#000000" SIZE=3 FAMILY="SANSSERIF" FACE="Arial" LANG="0">
<BR>
<BR>More great pictures, and complete protocols, for rat pups and fish larvae
<BR>respectively in the following:
<BR>
<BR> An automated double staining procedure for bone and cartilage.
<BR> Miller DM & Tarpley J. 1996. Biotechnic & Histochemistry. 71: 79-83
<BR>
<BR> Detection of mineralized structures in early stages of development of
<BR>marine
<BR> Teleostei using a modified alcian blue-alizarin red double staining
<BR>technique for
<BR> bone & cartilage.
<BR> Gavaia PJ, Sarasquete C & Cancela ML. 1999. Biotech Histochem. 75: 79-84
<BR>
<BR>Incidentally, I do recommend Biotechnic and Histochemistry, the journal of
<BR>the Biological Stain Commission. Its full of neat methods and ideas, its good
<BR>looking, and its cheap - and we all know HistoNetters are deeply into
<BR>bargains! See its website at:
<BR> www.louisville.edu/journal/biotech-histochem
<BR>Ah - Being of a sceptical disposition I've just checked the site, and bits of
<BR>it are damaged! Its pretty though, but the editor can be contacted by email
<BR>at:
<BR> biotech-histochem@louisville.edu
<BR>
<BR>And yes of COURSE I'm biased - being on the editorial board
<BR>Bye - Richard Horobin
<BR>
<BR></FONT><FONT COLOR="#000000" SIZE=2 FAMILY="SANSSERIF" FACE="Arial" LANG="0">Institute of Biomedical & Life Sciences, University of Glasgow
<BR><B>T direct 01796-474 480 --- E RichardWHorobin@aol.com</B>
<BR><I>"What should we expect? Everything."</I></FONT></HTML>
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