It was shown in the early days of gold labeling for EM that OsO4 will do
strange things to the silver in the silver enhanced gold so that sometimes
you will see the silver separating from the gold. ( I am sorry I do not
have a reference on hand. ) This is why Nanoprobes developed the
Goldenhance for their nanogold and larger particles.  Goldenhance works
very well and Osmium does not affect it.

At 08:37 AM 3/2/01 -0500, you wrote:
>ok a fairly simple question. we are using small gold prbes for localization 
>in vibratome sections. and then silver intensifing them. the problem is we 
>want to use them for EM. after we have OsO4 treated the tissue and embedded 
>and cut we find a loss of some (part o)f the silver. is there a way to 
>prevent this.
>john
>

Corazon D. Bucana, Ph.D.
Dept. Cancer Biology
UT M.D.Anderson Cancer Center
1515 Holcombe Blvd, Box 173
Houston, Texas 77030
Phone: 713-792-8106
FAX 713-792-8747