Penny,
I concur with Dana about different manufacturer's antibody diluents. Some antibodies of one company may not be stable with another's diluent. I had a problem with an actin antibody, that would break down in a few days and give a lot of background staining until I went back to using the same company's diluent.
This problem may not arise, if you only dilute what you will use that day, but I want to be able to store the diluted antibody at least a few weeks, if not months.
Katri

Katri Tuomala
Hamilton, Ontario, Canada

Penelope Marr wrote:

> Dana,
> What do you mean that diluents are not miscible???  They are all aqueous
> solutions of some sort - buffered, with a protein carrier, sodium azide and
> some have a blocking agent.  The detection systems are all made in similar
> solutions so how can any of these immiscible?
>
> Penny
>
> Penelope Marr
> Immunohistochemist
> S.E.A.L.S.
> St George Hospital
> Gray St
> Kogarah  NSW  2218
> Australia
>
> > -----Original Message-----
> > From: DDittus787@aol.com [SMTP:DDittus787@aol.com]
> > Sent: Tuesday, March 13, 2001 2:49 PM
> > To:   Aidan.Schurr@hvh.co.nz; histonet@pathology.swmed.edu
> > Subject:      Re: immunodiluent
> >
> > Dear Aidan
> > Over a very long period of time I have found diluents that are not
> > miscible ,
> > so to speak with certain companies detections, however if you use their
> > diluent you get the expected result, so we use our 3 main suppliers of
> > antibodies diluents.
> >
> >
> >  Dana
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