Re: immunodiluent

From:Katri Tuomala <>

I concur with Dana about different manufacturer's antibody diluents. Some antibodies of one company may not be stable with another's diluent. I had a problem with an actin antibody, that would break down in a few days and give a lot of background staining until I went back to using the same company's diluent.
This problem may not arise, if you only dilute what you will use that day, but I want to be able to store the diluted antibody at least a few weeks, if not months.

Katri Tuomala
Hamilton, Ontario, Canada

Penelope Marr wrote:

> Dana,
> What do you mean that diluents are not miscible???  They are all aqueous
> solutions of some sort - buffered, with a protein carrier, sodium azide and
> some have a blocking agent.  The detection systems are all made in similar
> solutions so how can any of these immiscible?
> Penny
> Penelope Marr
> Immunohistochemist
> S.E.A.L.S.
> St George Hospital
> Gray St
> Kogarah  NSW  2218
> Australia
> > -----Original Message-----
> > From: []
> > Sent: Tuesday, March 13, 2001 2:49 PM
> > To:;
> > Subject:      Re: immunodiluent
> >
> > Dear Aidan
> > Over a very long period of time I have found diluents that are not
> > miscible ,
> > so to speak with certain companies detections, however if you use their
> > diluent you get the expected result, so we use our 3 main suppliers of
> > antibodies diluents.
> >
> >
> >  Dana
> ******************************************************************************************************************************************************************************
> This email and the files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, you are not permitted to distribute or use this message or any of its attachments in any way. We also request that you advise the sender of the incorrect addressing.
> This note also confirms that this email message has been virus scanned and although no computer viruses were detected, South East Health accepts no liability for any consequential damage resulting from email containing any computer viruses.
> *****************************************************************************************************************************************************************************

<< Previous Message | Next Message >>