Re: immunodiluent

From:
DDittus787@aol.com

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By miscible I meant that there are chemical properties to some that do not 
jive" well with some detections and others that do, for example I used an 
automated manufacturers diluent with another companys antibody and got no 
staining, however when I did the same dilutions with the antibody 
manufacturers diluent and kept the same detection, I got staining, so somehow 
the diluent ( which was the only variable ) same case, same tissue , same 
detection, same machine................did not mix well with the detection.   
 Seen this in quite a few cases, lead me to do quite a bit of testing.        
                              Dana

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<HTML><FONT FACE=arial,helvetica><FONT  SIZE=2>By miscible I meant that there are chemical properties to some that do not 
<BR>jive" well with some detections and others that do, for example I used an 
<BR>automated manufacturers diluent with another companys antibody and got no 
<BR>staining, however when I did the same dilutions with the antibody 
<BR>manufacturers diluent and kept the same detection, I got staining, so somehow 
<BR>the diluent ( which was the only variable ) same case, same tissue , same 
<BR>detection, same machine................did not mix well with the detection.   
<BR> Seen this in quite a few cases, lead me to do quite a bit of testing.        
<BR>                              Dana</FONT></HTML>

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