Re: Staining/Processing problem

From:Mplhisto@aol.com


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   I hope that someone can help me out, we have had a problem a couple of 
times recently where  areas of the slide are not staining, the area looks  
like  the  slide did not deparaffinize good in spots. But I feel like the 
deparafinization step should be complete. The slides seem to be dried good, 2 
slides were even control slides that had been dried for a month or so. These 
both appeared at the beginning of the week, on relativley light workload 
days. These are stained on the Sakura stainer, and normally our slides are 
beautiful. Could the Xylene be contaminated?? We do use recycled. Please 
help!!!
         Meredith

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<HTML><FONT FACE=arial,helvetica><FONT  COLOR="#000040" SIZE=2>   I hope that someone can help me out, we have had a problem a couple of 
<BR>times recently where  areas of the slide are not staining, the area looks  
<BR>like  the  slide did not deparaffinize good in spots. But I feel like the 
<BR>deparafinization step should be complete. The slides seem to be dried good, 2 
<BR>slides were even control slides that had been dried for a month or so. These 
<BR>both appeared at the beginning of the week, on relativley light workload 
<BR>days. These are stained on the Sakura stainer, and normally our slides are 
<BR>beautiful. Could the Xylene be contaminated?? We do use recycled. Please 
<BR>help!!!
<BR>         Meredith</FONT></HTML>

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