Re: Staining/Processing problem
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I hope that someone can help me out, we have had a problem a couple of
times recently where areas of the slide are not staining, the area looks
like the slide did not deparaffinize good in spots. But I feel like the
deparafinization step should be complete. The slides seem to be dried good, 2
slides were even control slides that had been dried for a month or so. These
both appeared at the beginning of the week, on relativley light workload
days. These are stained on the Sakura stainer, and normally our slides are
beautiful. Could the Xylene be contaminated?? We do use recycled. Please
help!!!
Meredith
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<HTML><FONT FACE=arial,helvetica><FONT COLOR="#000040" SIZE=2> I hope that someone can help me out, we have had a problem a couple of
<BR>times recently where areas of the slide are not staining, the area looks
<BR>like the slide did not deparaffinize good in spots. But I feel like the
<BR>deparafinization step should be complete. The slides seem to be dried good, 2
<BR>slides were even control slides that had been dried for a month or so. These
<BR>both appeared at the beginning of the week, on relativley light workload
<BR>days. These are stained on the Sakura stainer, and normally our slides are
<BR>beautiful. Could the Xylene be contaminated?? We do use recycled. Please
<BR>help!!!
<BR> Meredith</FONT></HTML>
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