I developed a Cresyl Fast Violet Acetate stain for
mast cells which remains metachromatic after
differentiation in acid alcohol and dehydration.  The
slides I stained in 1974 have hardly faded at all.

The method sounds a little strange but it does work!


Stain hot 2% CFV in glycerol about 60C for 10 mins,

Wash in warm water,

Check microscopically,
if the staining is too weak then stain for longer,

When the section looks overstained differentiate in
acid alcohol, check microscopically, metachromatic
substances red/dark-pink, nuclei blue and background
colourless.

Dehydrate in alcohol, clear and mount.

If you can't get the section to stain darkly enough
then the glycerol is fresh and hasn't absorbed water
from the air so dilute a little with water.

The dye takes a day or two to dissolve in the glycerol
and takes on a lovely fluorescent red colour.

Its better than a Geimsa because you can diff' out the
background with this method.

If you try it let me know how you get on.

Best Wishes

Steve Machin UK



> > > 
> > > Hi,
> > > It might be a really basic and naive question
> but does any one knows what
> > > HEMO-D is ? (xylene substitute?), 
> > > who might be the suppliers?
> > > I am after a protocol for neuronal tissue
> staining Cresyl violet.
> > > 
> > > Any help is greatly appreciated.
> > > 
> > > 
> > > Best Regards
> > > 
> > > 
> > > Tarik Khaznadar
> > > Vernalis Research Limited
> > > Reading
> > > U.K
> > > 
> > > vernalis.com
> > > 
> > > 
> > > 
> > > .
> > 
> > .
> 
> 


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