Responding to the message of <412A3799F627D31191D40008C7DB7BBED80B70@SERVER3>
from "Khaznadar, Tarik" <T.Khaznadar@vernalis.com>:

Here is the cresy violet stain we use. I also have a protocol which calls for a 
1% cresyl violet solution, and increased staining time to 20-30 minutes, then 
differentiation in 95% alcohol until most of the stain is removed. The following
method works well for us so it is the one I use routinely. Hope this helps. 
LuAnn

Cresy Violet for Nissl Staining

Purpose:
The identification of nuerons in tissue sections or the demonstration of the 
loss of nissl substaince. This is useful in determining neuronal damage.

Principle:
Neurons contain Nissl substance, which is primarily composed of rough 
endoplasmic reticulum, with the amount, form and distributioin varying in 
different types of neurons. Because of RNA content, Nissl substance is very 
basophilic and will be sharply stained with basic aniline dyes.

Quality Control:
A section of spinal cord is a good control.

Reagents:

Cresyl Violet..............5 gm
Distilled water...........100 cc.
ripen 24-48 hours. Filter.

Method:
1. Deparaffinize well and hydate to water.
2. Stain 3-5 minutes in Cresy Violet solution.
3. Rinse in 2 changes of distilled water.
4. Quick dip in acid alcohol. Put immediately into water to stop     
   differentiation.
5. Wash in running tap water.
6. Dip quickly in 95% alcohol, then absolute alcohol.
7. Xylene-several changes. Mount in permount.

Results:
Nissl substance...purple.

Reference:
Keller, G.T.  AFIP
> 
> That's right. This will enable me to visualise the track of the injection
> needle, hence check that my stereotaxic injection are correctly positioned.
> Regards
> 
> -----Original Message-----
> From: LuAnn Anderson [mailto:ander093@gold.tc.umn.edu]
> Sent: 28 February 2001 18:01
> To: T.Khaznadar@vernalis.com
> Cc: Histonet@pathology.swmed.edu
> Subject: Re: Query
> 
> 
> Responding to the message of
> <412A3799F627D31191D40008C7DB7BBED80B6D@SERVER3>
> from "Khaznadar, Tarik" <T.Khaznadar@vernalis.com>:
> 
> Are you looking to stain Nissl substance??
> 
> 
> 
> 
> > 
> > Hi,
> > It might be a really basic and naive question but does any one knows what
> > HEMO-D is ? (xylene substitute?), 
> > who might be the suppliers?
> > I am after a protocol for neuronal tissue staining Cresyl violet.
> > 
> > Any help is greatly appreciated.
> > 
> > 
> > Best Regards
> > 
> > 
> > Tarik Khaznadar
> > Vernalis Research Limited
> > Reading
> > U.K
> > 
> > vernalis.com
> > 
> > 
> > 
> > .
> 
> .