Re: Please help to solve the problem with immunostaining of melanoma samples

From:DDittus787@aol.com


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What are you using to dilute your fluid for titer?, Also I do not know if it 
is the IgM, versus the IgG or not, however certain melanomas do carry high 
backgrounds due to necrosis, etc. Could it possibly be an unclean secondary?
                                                       Dana

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<HTML><FONT FACE=arial,helvetica><FONT  SIZE=2>What are you using to dilute your fluid for titer?, Also I do not know if it 
<BR>is the IgM, versus the IgG or not, however certain melanomas do carry high 
<BR>backgrounds due to necrosis, etc. Could it possibly be an unclean secondary?
<BR>                                                       Dana</FONT></HTML>

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