Re: PFA perfusion

From:Dimitri Scholz <>

Dear Veronique,

I got some experience wit PFA perfusion fixation. First of all, you 
better reduce its concentration to 1-2 % because it smells. Secondly, 
reduced concentration of PFA allows better immunohistochemistry. No 
worry, Beta-gal reaction goes even after strong fixation. Usually, I 
postfix tissues for some hours or overnight in same fix-solution.

Bon chance!

Dr. Dimitri Scholz              |  voice    ++49 6032 705-403
Max-Planck-Institute            |  Fax      ++49 6032 705-419
Dept. of Exp. Cardiology        |  Email
Beneke-Str.2                    |
D-61231 Bad Nauheim             |
Germany                         |

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