Re: PFA perfusion
From: | Dimitri Scholz <d.scholz@kerckhoff.mpg.de> |
Dear Veronique,
I got some experience wit PFA perfusion fixation. First of all, you
better reduce its concentration to 1-2 % because it smells. Secondly,
reduced concentration of PFA allows better immunohistochemistry. No
worry, Beta-gal reaction goes even after strong fixation. Usually, I
postfix tissues for some hours or overnight in same fix-solution.
Bon chance!
Dimitri
--
Dr. Dimitri Scholz | voice ++49 6032 705-403
Max-Planck-Institute | Fax ++49 6032 705-419
Dept. of Exp. Cardiology | Email d.scholz@kerckhoff.mpg.de
Beneke-Str.2 |
D-61231 Bad Nauheim |
Germany |
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