Re: Methylene Blue eosinate

From:Connie McManus <>

I read your message, but I don't think it is Jenner's stain because in
the procedure, Jenner's Stain is used in the step preceding this mystery
concoction. Like I said before, I think this is the actual formulation
for Giemsa stain.  I found the exact same recipe in a 1947 edition of RD
Lillie's Histochemistry techniques under "Giemsa stain" and he describes
how to make up the Giemsa stain.  Thus, I am left with skipping this old
version and making up giemsa stain from my good new stuff from Sigma.

thanks, anyway, bert!  
gotta run


Bert Dotson wrote:
> Connie, you must not have seen my post that methylene blue eosinate would
> be a relatively appropriate name for the stain currently called Jenner's
> Stain. It is commonly used in Giemsa variations. It is a salt of the
> methylene blue cation and the eosin Y anion. The term "eosinate" may be a
> little suspect but makes sense in this context.
> Bert
> -----Original Message-----
> From:   Connie McManus []
> Sent:   Monday, March 05, 2001 1:26 PM
> To:     Geoff McAuliffe
> Cc:     Histonet
> Subject:        thanks re Methylene Blue eosinate
> I have come to the conclusion that my old procedure is probably just a
> Giemsa stain (we're staining for protozoan parasites), so that's what
> I'm going to do.  To date, no one has been able to tell me just what
> methylene blue eosinate is, so I'm assuming it is such an ancient dye
> that it hasn't been produced in 90 years OR it has a completely
> different name and isn't traced back to this older usage.  At any rate,
> this has been a real puzzler and I thank you all for trying your
> collective best to help me.
> Connie M


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