Re: Methylene Blue eosinate
|From:||Connie McManus <firstname.lastname@example.org>|
I read your message, but I don't think it is Jenner's stain because in
the procedure, Jenner's Stain is used in the step preceding this mystery
concoction. Like I said before, I think this is the actual formulation
for Giemsa stain. I found the exact same recipe in a 1947 edition of RD
Lillie's Histochemistry techniques under "Giemsa stain" and he describes
how to make up the Giemsa stain. Thus, I am left with skipping this old
version and making up giemsa stain from my good new stuff from Sigma.
thanks, anyway, bert!
Bert Dotson wrote:
> Connie, you must not have seen my post that methylene blue eosinate would
> be a relatively appropriate name for the stain currently called Jenner's
> Stain. It is commonly used in Giemsa variations. It is a salt of the
> methylene blue cation and the eosin Y anion. The term "eosinate" may be a
> little suspect but makes sense in this context.
> -----Original Message-----
> From: Connie McManus [SMTP:email@example.com]
> Sent: Monday, March 05, 2001 1:26 PM
> To: Geoff McAuliffe
> Cc: Histonet
> Subject: thanks re Methylene Blue eosinate
> I have come to the conclusion that my old procedure is probably just a
> Giemsa stain (we're staining for protozoan parasites), so that's what
> I'm going to do. To date, no one has been able to tell me just what
> methylene blue eosinate is, so I'm assuming it is such an ancient dye
> that it hasn't been produced in 90 years OR it has a completely
> different name and isn't traced back to this older usage. At any rate,
> this has been a real puzzler and I thank you all for trying your
> collective best to help me.
> Connie M
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