Re: Immunohistochemistry on brain sections

From:Amos & Theresa <>

    Since you are using formic acid on brain I assume you are staining for Beta
Amyloid, right? (Just a guess) We use the same procedure, but we do not
autoclave the slides. I'd try to eliminate one or the other. I have found that
compound retrieval techniques are usually harsh on the tissues, and formic acid
alone is harsh even with out the other procedures. If it is Beta Amyloid you are
staining we get good results with just formic acid. Let me know if I could help
Amos Brooks wrote:

> Hi Histonetters,
> I am having difficulty with brain sections adhering to the slides during
> Immunohistochemistry, even using the Aminosilane-coated slides, dried
> overnight at 60 degrees. These sections are hydrated, placed in a 96% formic
> acid bath for 5 mins. followed by autoclaving for 25 mins. The slides are
> then stained with the Dako Autostainer.  Any suggestions would be greatly
> appreciated.
> Thanks in advance.
> Marilyn Johnson
> Agri-Foods Surveillance Systems
> Alberta Agriculture
> Edmonton, AB.

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