Re: Immunohistochemistry on brain sections
From: | Amy Porter <portera203@yahoo.com> |
Marilyn - you could try letting your slides air dry
prior to placing them in the oven to dry. We dry all
of our neuropath slides overnight in a high air flow
vent or hood, then place them in the oven overnight.
Hope this helps.
--- marilyn.johnson@gov.ab.ca wrote:
> Hi Histonetters,
> I am having difficulty with brain sections adhering
> to the slides during
> Immunohistochemistry, even using the
> Aminosilane-coated slides, dried
> overnight at 60 degrees. These sections are
> hydrated, placed in a 96% formic
> acid bath for 5 mins. followed by autoclaving for 25
> mins. The slides are
> then stained with the Dako Autostainer. Any
> suggestions would be greatly
> appreciated.
> Thanks in advance.
>
> Marilyn Johnson
> Agri-Foods Surveillance Systems
> Alberta Agriculture
> Edmonton, AB.
>
>
>
=====
Amy S.Porter, HT(ASCP)
Michigan State University
College of Human Medicine
portera203@yahoo.com
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