Re: Immunohistochemistry on brain sections

From:Amy Porter <>

Marilyn - you could try letting your slides air dry
prior to placing them in the oven to dry. We dry all
of our neuropath slides overnight in a high air flow
vent or hood, then place them in the oven overnight. 
Hope this helps.  
--- wrote:
> Hi Histonetters,
> I am having difficulty with brain sections adhering
> to the slides during
> Immunohistochemistry, even using the
> Aminosilane-coated slides, dried
> overnight at 60 degrees. These sections are
> hydrated, placed in a 96% formic
> acid bath for 5 mins. followed by autoclaving for 25
> mins. The slides are
> then stained with the Dako Autostainer.  Any
> suggestions would be greatly
> appreciated.
> Thanks in advance.
> Marilyn Johnson
> Agri-Foods Surveillance Systems
> Alberta Agriculture
> Edmonton, AB.

Amy S.Porter, HT(ASCP)
Michigan State University
College of Human Medicine

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