Re: Immunohistochemistry on brain sections

From:Geoff McAuliffe <mcauliff@UMDNJ.EDU> wrote:

> Hi Histonetters,
> I am having difficulty with brain sections adhering to the slides during
> Immunohistochemistry, even using the Aminosilane-coated slides, dried
> overnight at 60 degrees. These sections are hydrated, placed in a 96% formic
> acid bath for 5 mins. followed by autoclaving for 25 mins. The slides are
> then stained with the Dako Autostainer.  Any suggestions would be greatly
> appreciated.
> Thanks in advance.
> Marilyn Johnson
> Agri-Foods Surveillance Systems
> Alberta Agriculture
> Edmonton, AB.

Can you do the immuno stain on free-floating sections, then mount them on

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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