Re: H&E of decalcified bone samples

From:Katri Tuomala <katri@istar.ca>

Hi David,
You can improve hematoxylin staining on decalcified sections by just increasing
the staining time. For instance, if our regular staining time in Mayer's
hematoxylin is 8 min., we stain bones left long in acid (6 to 10 days or longer)
maybe 16 minutes. So double the time you normally stain in hematoxylin. Good
luck!
Katri

Katri Tuomala
Hamilton, Ontario, Canada

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