Re: H&E of decalcified bone samples

From:Katri Tuomala <>

Hi David,
You can improve hematoxylin staining on decalcified sections by just increasing
the staining time. For instance, if our regular staining time in Mayer's
hematoxylin is 8 min., we stain bones left long in acid (6 to 10 days or longer)
maybe 16 minutes. So double the time you normally stain in hematoxylin. Good

Katri Tuomala
Hamilton, Ontario, Canada


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