Re: Endothelial Markers

From:Barbara Wright <bwright@gene.COM>


Both CD31 and CD34 work well in formalin fixed paraffin embedded tissues.  I
get my CD31 from DAKO clone JC/70A and my CD34 from NovoCastro clone
QBEnd/10.  I use HEIR - preheated DAKO Target Retrieval at 99C and then
incubate my section in the preheated solution for 20 minutes followed by
immediate rinse in PBS.  CD31 needs tyramide amplification to really get
good intense staining.   If you need help please don't hesitate to email me
back.  Good luck

Barb Wright

Karen M Majahad wrote:

> I am trying find some good human endothelial markers for tissues that
> are fixed in formalin and embedded in paraffin. I have tryed Von
> Willebrand Factor but it does not stain all the endothelial cells. I was
> wondering if anyone has tryed  markers for CD31, CD34, or collagen type
> IV. If anyone has had good luck please e-mail me the manufacture of the
> antibody and the procedure.

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