|From:||Luis Chiriboga <Luis.Chiriboga@med.nyu.edu>|
I believe you need to make an extract, either directly or by lightly wetting the
substrate and then doing a touch prep from the substrate onto a glass slide.
From there you can do the "christmas tree stain" which will demonstrate
spermatozoa but is not conclusive for spermatozoa. I do not recall the recipe
for it has been a number of years since I have done.
I suggest you contact Dr. Lawrence Kobilinsky. He is the associate provost at
the city university of new york, John jay college of criminal justice and an
experiment in forensic biology.
> To all,
> The problem we have in the forensic field is that nothing is constant, we
> work with stains from clothes and slides made from the vagina ect.
> These stains are not fresh(mostly more than 3 days old) and therefore very
> vonurable for extern invirement.
> Some even have alot of yeast cells(like from the anus), and if sperm loses
> there tail, it is some times difficult to differntiate between spermcells
> and yeastcells.
> It is very important to diagnose if there is sperm or not(in a court of law)
> On the other hand the stain canot take to long, since we have alot of these
> cases(daily) and we can't spend much time on a case.
> We are testing acridine orange stains at the moment but I can't get
> satisfactorry results, I searched for relevant articels and book in the
> forensic field but can't find nothing.
> Hoping you can give me an advise,
> Sincerly Yours,
> Brinkenberg MNA
Luis Chiriboga Ph.D, H.T. (ASCP) QIHC
New York University School of Medicine
Department Of Pathology 4W27, Bellevue Hospital
27st & First Avenue
New York, NY 10016
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