Re: Anti-SMA in mouse

From:Barbara Wright <bwright@gene.COM>


I do Actin Smooth Muscle in 10% NBF paraffin embedded mouse tissues. I use
DAKO Clone 1A4 at 1:800.  No antigen retrieval is necessary.  I use Southern
Biotechnology's biotinylated goat anti-mouse IgG2a diluted 1:100 as my
secondary reagent.  Followed by Vectastain Elite ABC and DAB chromogen. Hope
that helps.

Barb Wright :) wrote:

> Help me! Does anyone do anti-SMA staining in mouse tissues? What antibody
> should be used and how should the tissues be prepared?
> Andrea

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