Rick:

I have often soaked blocks in ice water overnight with no problems at all.
I have heard of mouse tissue popping out of blocks, but have never
experienced that myself.

Jeanine Bartlett, HT(ASCP)
Centers for Disease Control
1600 Clifton Rd. NE
Mailstop G-32
Atlanta, GA  30333
jbartlett@cdc.gov 





-----Original Message-----
From: Rick Haverland [mailto:corycody@msn.com]
Sent: Wednesday, March 14, 2001 7:19 AM
To: histonet@pathology.swmed.edu
Subject: soaking blocks


I would like to get some input on the appropriate amount of time to 
soak/chill animal tissue before cutting it. In my experience, properly 
processed tissue takes a very short amount of time before it is ready to 
cut. The lab I am currently working for is recommending soaking blocks 
overnight on ice in a refrigerator. I have never heard of this and am 
concerned about tissue damage. Thanks for your help.

Bly Haverland
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