Andrea
	We have tried a few techniques for this, the results are worth the
extra effort if you want a dual stain of bone and cartilage, the best was
A procedure for differential staining of cartilage and bone in whole
formalin fixed vertebrates
Richard J Wasserug
Stain Technology Vol 51 No2 1976

If you can't get this, or want other references to variants of the technique
I can supply more details

Best of luck
	Hamish

-----Original Message-----
From: Andrea Grantham [mailto:algranth@u.arizona.edu]
Sent: 14 March 2001 00:10
To: histonet@pathology.swmed.edu
Subject: procedure staining for bone and cartilage


I was wondering if anybody does or has done a stain for bone and cartilage 
on mouse pups using Alizarin Red S and Alcian Blue. The investigator 
brought a great picture in a book and asked me to do this stain but I can't 
find a procedure.
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
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