RE: Washed Out Nuclei

From:Kari Dalziel <>

At one point we had a similiar problem.  We found that we actually had 
xylene contamination in the processor.  We were using a fixative that 
needed to be made up with alcohol and one of the assistants accidentally 
made it up with xylene.  The weird thing was not all of the tissues were 
affected.  I would look for xylene contamination during some part of your 
processing schedule.

-----Original Message-----
From: []
Sent:	Tuesday, March 13, 2001 11:04 AM
Subject:	Washed Out Nuclei

Can anyone tell me why, intermittently, we are having slides that appear to 
have washed out nuclear staining.  Mostly on endometrium, sometimes on
reprocessed skin.  Everything is processed/stained/etc. together.  Does
anyone have a good reprocessing schedule?  We use VIP tissue processors.

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