Try putting some more rat serum in your blocking step. I block for 20 min.
with a 10% solution of serum from the host of the secondary which has added
to it about 1% rat serum and I use this for 20 min. before the primary
antibody and then again for 5 min. before the secondary.
Sent: Tuesday, March 13, 2001 4:44 PM
I'm using a CD3 marker grown in mice that cross reacts with
I'm getting a lot of background staining in
axillary tissue on the slides.(mainly heart.) Doe anyone
suggestions to eliminate this backround staining. I'm using
a Vector Elite Peroxidase kit and my CD3 marker is made by
staining rat tissue. and I have already tried Casein(Sigma),
Power Block(BioGenex), an anti-mouse IgG rat adsorbed
as the secondary antibody. All help is much appreciated.
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