|From:||Roy Ellis <email@example.com>|
After incubation you should rinse in distilled water then mount under a
coverslip using an aqueous mounting medium. When the medium sets, seal the
coverslips with clear nail varnish.
A reference is Bonilla E, Schotland DL. Histochemical diagnosis of muscle
phosphofructokinase deficiency. Arch Neurol 1970;22:8-12
> -----Original Message-----
> From: Klassen, Shannon SDH [mailto:firstname.lastname@example.org]
> Sent: Friday, 2 March 2001 5:53
> To: 'Histonet'
> Help I need a method for phosphofructokinase for frozen muscle
> tissue - my
> method isn't working well and the reference is very old and I don't have
> access to it.
> Or is there a enzyme genius out there who can help me?
> Stain done on freshly cut frozen sections - no fixation - go
> straight into incubation media for 1 hour @ 37C. Rinse thru 30% and 60%
> acetone and then distilled water. Everything appears good up to
> this point.
> Then dehydrate thru alcohols and xylene - this is where the reaction fades
> to almost nothing. Should I just leave them in water and mount with an
> aqueous mount?????? I don't really want to play around with the stain too
> much as the chemicals can be a little pricey.
> Thanks for the help in advance. Shannon
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