From:Roy Ellis <>

After incubation you should rinse in distilled water then mount under a
coverslip using an aqueous mounting medium. When the medium sets, seal the
coverslips with clear nail varnish.
A reference is Bonilla E, Schotland DL. Histochemical diagnosis of muscle
phosphofructokinase deficiency. Arch Neurol 1970;22:8-12
Roy Ellis

> -----Original Message-----
> From: Klassen, Shannon SDH []
> Sent: Friday, 2 March 2001 5:53
> To: 'Histonet'
> Subject:
> Help  I need a method for phosphofructokinase for frozen muscle
> tissue - my
> method isn't working well and the reference is very old and I don't have
> access to it.
> Or  is there a enzyme genius out there who can help me?
> 	Stain done on freshly cut frozen sections - no fixation - go
> straight into incubation media for 1 hour @ 37C.  Rinse thru 30% and 60%
> acetone and then distilled water.  Everything appears good up to
> this point.
> Then dehydrate thru alcohols and xylene - this is where the reaction fades
> to almost nothing.  Should I just leave them in water and mount with an
> aqueous mount??????  I don't really want to play around with the stain too
> much as the chemicals can be a little pricey.
> Thanks for the help in advance.  Shannon

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