Immunohistochemistry on brain sections

Hi Histonetters,
I am having difficulty with brain sections adhering to the slides during
Immunohistochemistry, even using the Aminosilane-coated slides, dried
overnight at 60 degrees. These sections are hydrated, placed in a 96% formic
acid bath for 5 mins. followed by autoclaving for 25 mins. The slides are
then stained with the Dako Autostainer.  Any suggestions would be greatly
Thanks in advance.

Marilyn Johnson
Agri-Foods Surveillance Systems
Alberta Agriculture
Edmonton, AB.

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