Immunohistochemistry on brain sections

From:marilyn.johnson@gov.ab.ca

Hi Histonetters,
I am having difficulty with brain sections adhering to the slides during
Immunohistochemistry, even using the Aminosilane-coated slides, dried
overnight at 60 degrees. These sections are hydrated, placed in a 96% formic
acid bath for 5 mins. followed by autoclaving for 25 mins. The slides are
then stained with the Dako Autostainer.  Any suggestions would be greatly
appreciated.
Thanks in advance.

Marilyn Johnson
Agri-Foods Surveillance Systems
Alberta Agriculture
Edmonton, AB.





<< Previous Message | Next Message >>