IHC on kidney -Reply

From:Tony Henwood <AnthonyH@chw.edu.au>


Beware kidney is rich in biotin, so blocking is probably a good idea. AND
keep a careful eye on your negative controls.

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> "Bennett, Catherine (Katie)" <cbennett@lrri.org> 13/March/2001
04:46am >>>
I have never done IHC staining on kidney tissue before and was
wondering if
an avidin/biotin blocking step is needed and/or useful.

Catherine "Katie" Bresee Bennett
Sr. Research Technologist
Lovelace Respiratory Research Institute
Albuquerque, New Mexico

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