DAB -Reply

From:Tony Henwood <AnthonyH@chw.edu.au>

Yes, you are right. The DAB product will remain. It has a high
substantivity for tissue elements.
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

>>> Luis Chiriboga <Luis.Chiriboga@med.nyu.edu> 2/March/2001
04:16am >>>
Thanks to all of you who replied to my IHC question. However,  I think I
may have asked the question the wrong way.   I guess what I really
wanted to know was if there is a way to remove the DAB from the
I imagine that the antibodies can be eluted off by washing the slides in
a salt solution of high ionic strength,  similar to chromatographic
methods.  I presume this will leave the DAB precipitate in place?
Any thoughts on this???

Luis Chiriboga Ph.D, H.T. (ASCP) QIHC
New York University School of Medicine
Department Of Pathology 4W27, Bellevue Hospital
27st & First Avenue
New York, NY 10016

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