Alcohol fixation for EDTA decalcification
|From:||Hofecker Jennifer <Jennifer_Hofecker@URMC.Rochester.edu>|
I am going to receive some mouse legs which have been "fixed" in alcohol
(unsure of percent, I assume 70%). I need to separate the femur from the
tibia and process the femur.
I use 10% EDTA for decalcification. My question is what should I expect
when I put the femur right into EDTA from alcohol? I normally fix anything
going through for paraffin in formalin.
Thanks in advance,
Jennifer Hofecker, HT (ASCP)
University of Rochester
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