Alcohol fixation for EDTA decalcification

From:Hofecker Jennifer <Jennifer_Hofecker@URMC.Rochester.edu>

Hi Everyone,
I am going to receive some mouse legs which have been "fixed" in alcohol
(unsure of percent, I assume 70%).  I need to separate the femur from the
tibia and process the femur.
I use 10% EDTA for decalcification.   My question is what should I expect
when I put the femur right into EDTA from alcohol?  I normally  fix anything
going through for paraffin in formalin.

Thanks in advance,
Jennifer

Jennifer Hofecker, HT (ASCP)
University of Rochester
Pathology
(716)273-4129



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