Alcohol fixation for EDTA decalcification

From:Hofecker Jennifer <>

Hi Everyone,
I am going to receive some mouse legs which have been "fixed" in alcohol
(unsure of percent, I assume 70%).  I need to separate the femur from the
tibia and process the femur.
I use 10% EDTA for decalcification.   My question is what should I expect
when I put the femur right into EDTA from alcohol?  I normally  fix anything
going through for paraffin in formalin.

Thanks in advance,

Jennifer Hofecker, HT (ASCP)
University of Rochester

<< Previous Message | Next Message >>