I use a variation on Gayle's method. I buy Mayer's modified hematoxylin
from Polyscience, Cat# s216, dilute it 1/40 with distilled water and stain
for 3 minutes, then blue for 1 minute in homemade Scott's tap water. The
result is a very nice light blue counterstain which contrasts beautifully
with DAB. It is only good for 1 day also but is very cheap to make this way!

Mary Ann
Mary Ann Miller
University of Cincinnati
College of Medicine  ML 529
231 Bethesda Avenue
Cincinnati, Ohio  45267
FAX: (513) 558-2289