fixation of ND10 in tissue
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From: | xyz@wistar.upenn.edu (Zhao, Xinyu) |
To: | HistoNet@Pathology.swmed.edu |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Dear everybody there,
I am trying to do immunohistochemical staining with mouse liver tissues to
see ND10 (Nuclear Domain 10), which are some dots in the nuclears. I have
done a couple of test, but it seems that the fixation was not done properly
as there were no dots left, although my positive control for the staining
procedure worked fine.
I wonder if anybody outthere has had any experience in working with these
nuclear domain dots in tissue and could shed some light on how I can
preserve them and so on.
Any input will be very much appreciated.
Jasmine
The Wistar Institute
Philadelphia
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