cold perfusion
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From: | "Philopena, Jennifer" <jennifer.philopena@canji.com> |
To: | 'Histonet' <histonet@pathology.swmed.edu> |
Reply-To: | |
Content-Type: | text/plain; charset="windows-1252" |
I worked at Cornell years ago and while there I would perfuse the whole rat
with ice cold paraformaldehyde (we were taking only the brain for analysis),
but I would pre-perfuse with a heparin/sodium nitrate solution (I forgot the
concentration) to dilate the blood vessels. The brains were sucrose loaded
(30%) and cryosectioned. They looked beautiful. Jen
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