citrate buffer recipe

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From:"Sebree Linda A." <>
To:'Histonet' <>, 'IPOX' <>

Thanks to all of you that responded to my request for citrate buffer
recipes.  The responses I received fell into 2 categories:  10mM citrate
buffer using citric acid only and 1mM citrate buffer employing citric acid
and sodium citrate.  My question is:  since the 1 ingredient buffer can be
pH'd in its stock form and is stable for months even at room temperature is
there any good reason to use the 2 ingredient buffer that needs to be pH'd
in its working form and calls for mixing the 2 solutions at the time of use?

My common sense tells me the citric acid only buffer is easier and less
fussy.  But I will consider the citric acid/sodium citrate buffer if there
are compelling reasons to use it instead, ie it works better, is gentler on
tissue, intensifies staining more, etc.

Your experienced input is greatly appreciated,


P.S. Has anyone observed differences or done a comparison of either of these
buffers with commercially available citrate buffers?    

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
600 Highland Avenue
Madison, WI  53792-2472

FAX: (608)263-1568

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