biebrich scarlet-acid fuschin

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From:Jay Turner <>
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I am staining de-calcified bone with a trichrome stain of iron hematoxylin, 
aniline blue and biebrich scarlet-acid fuschin.  I am getting varied results 
from bone to bone.  The aniline is a collagen stain, but what are the 
actions of the biebrich scarlet?  I am getting  cortical bone to stain all 
blue, all red and mixtures of the two.  Can somebody who has done this work 
help me?

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