X-gal solution vs. anti-Beta-galactosidase Antibody

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From:"Patterson, Noelle" <PattersonN@NMRIPO.NMRI.NNMC.NAVY.MIL>
To:Histonet <HistoNet@Pathology.swmed.edu>

Hello Histonet,

I have been reading all of the cross talk about Beta galactosidase detection
with interest.  I have recently been tasked to detect beta galactosidase in
murine tissue.  Of course, I haven't done this before (well, I have done it
in bacterial colonies back in my molecular days).  

I am curious.  Those of you who have to detect this reagent, do you prefer
the antibody detection, or the enzyme development method for tissues?  What
are your reasons for such preference?  Please include all the pro's and
con's for each method, as your time allows.  

I am running my first experiment to detect this today (using the X-gal
solution detection method).  Wish me luck!

Another question:  Is there a commercial (or otherwise readily available)
source for beta galactosidase positive control blocks?  If so, would you
give me the ordering information?

Thanks for your time!

Noelle Patterson, M.S.
Bethesda, Md.  20889

stressed is desserts spelled backwards.  

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