I am staining a fractured rat femur with an immunohistochemical stain to 
IGF-1.  The stain is a standard biotin-avidin peroxidase with DAB.   The 
samples were decalcified in Cal-Ex (Fisher), it was a little harsh.  There 
is stain in the section but it is very faint.  I don't want to re-do these 
animals and re-decalcify.  I have tried to use a metal enhanced DAB (cobalt) 
  but the color is too close to the hematoxylin counterstain.  Is there any 
other way to enhance DAB and the staining?  Any suggestions?  Is 
fluorescence the way to go?

Thanks!

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